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Chatelier, Aurelien; Mckenzie, David; Prinet, Aurelie; Galois, Robert; Robin, Jean; Zambonino, Jose-luis; Claireaux, Guy. |
Seabass were fed for 4 months with diets where the lipid was provided as either canola oil (CO), palm oil ( PO) or fish oil (FO), to generate diversity in their tissue fatty acid (FA) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. In particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (OA, 18:1n-9 and LA, 18:2n-6, respectively) from the CO and PO diets would improve maximum exercise and cardiac performance, and increase aerobic metabolic scope. This proved to be the case; exercise respirometry on bass fitted with cardiac flow probes revealed that those fed CO and PO diets had a... |
Tipo: Text |
Palavras-chave: Diet; Fatty acid; Cardiovascular performance; Metabolism; Swimming; Dicentrarchus labrax; Seabass. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1860.pdf |
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Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; Mckenzie, David; Bois, P. |
The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage... |
Tipo: Text |
Palavras-chave: Fatty acid; Sea bass; Ventricular myocyte; L type calcium channel; Oleic acid. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2120.pdf |
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Claireaux, Guy; Mckenzie, David; Genge, A; Chatelier, Aurelien; Aubin, Joel; Farrell, Anthony. |
We exploited the inherent individual diversity in swimming performance of rainbow trout Oncorhynchus mykiss to investigate the hypothesis that maximum cardiac performance is linked to active metabolic rate (AMR) and critical swimming speed (U-crit). Six hundred juveniles (body mass similar to 150 g) were screened using a swimming challenge of 1.2 m s(-1) to identify 'poor swimmers' and 'good swimmers', i.e. the first and last 60 fish to fatigue, respectively. These 120 fish were individually tagged and then reared in common tanks for 9 months, where they grew at similar rates and achieved a similar body mass of approximately 1100 g. Critical swimming speed (U-crit) was then measured individually in tunnel respirometers, with simultaneous recordings of... |
Tipo: Text |
Palavras-chave: Cardiac anatomy; Cardiac rythm; Swimming activity; Trout. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-420.pdf |
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